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ObjectiveThrough the targeted lipidomics， we explored the intervention mechanism of Kaixuan Bushen method on psoriasis vulgaris （PV） from the perspective of lipid metabolism， providing reference for the diagnosis and treatment of PV. MethodTwenty-six patients with PV admitting the outpatient clinic of the Department of Dermatology， Guang'anmen Hospital， China Academy of Chinese Medical Sciences from September 2019 to November 2020 were selected as the research object （observation group）， and 26 sex- and age-matched healthy volunteers in the same period were recruited as control group. Venous blood was collected for lipid index and targeted lipidomics detection in the control and observation groups at inclusion. After 12 weeks of continuous treatment of Kaixuan Bushen method， the psoriasis area and severity index （PASI） was measured and compared before and after treatment to assess the clinical efficacy， while venous blood was collected again in the observation group to compare the blood lipid level and lipid metabolism of patients before and after treatment. Targeted lipidomics analysis was performed by ultra performance liquid chromatography tandem mass spectrometry （UPLC-MS/MS） on an ACQUITY UPLC BEH C8 column （2.1 mm×100 mm， 1.7 μm） with mobile phase of 5 mmol∙L-1 ammonium formate in acetonitrile-water （6∶4， A）-5 mmol∙L-1 ammonium formate in acetonitrile-isopropanol （1∶9， B） for gradient elution and flow rate of 0.26 mL∙min-1. Conditions of MS were electrospray ionization （ESI）， positive and negative ion modes， and scanning range of m/z 50-1 200. Then principal component analysis （PCA） and partial least squares-discriminant analysis （PLS-DA） models were developed to screen differential metabolites， and the differential metabolites were identified and the pathways were enriched. ResultAfter 12 weeks of treatment with Kaixuan Bushen method， PASI score decreased by more than 50% in a total of 22 out of 26 patients with PV， suggesting the total effective rate was 84.62%. The serum triglyceride level of patients with PV was significantly higher than that of healthy individuals （P<0.05）， and the triglyceride level was significantly reduced after treatment （P<0.05）. Targeted lipidomics analysis screened a total of 43 potential biomarkers for PV， of which 42 were up-regulated and 1 was down-regulated， involving 7 signaling pathways such as linoleic acid metabolism， glycerophospholipid metabolism and unsaturated fatty acid biosynthesis. Moreover， there were 14 response makers for clinical efficacy of Kaixuan Bushen method on PV， of which 6 were up-regulated and 8 were down-regulated， involving five signaling pathways such as linoleic acid metabolism， glycerophospholipid metabolism and sphingolipids metabolism. In a comparison between healthy individuals and patients with PV and PV before and after treatment， the common differential metabolites were screened as phosphatidylcholine （PC） 38∶0 and ceramide （Cer） 42∶1， and the common pathways were linoleic acid and glycerophospholipid metabolic pathways. ConclusionThe disorder of lipid metabolism in PV are largely due to abnormal sphingolipid， glycerophospholipid and linoleic acid metabolic pathways， of which Kaixuan Bushen method can regulate the glycerophospholipid and linoleic acid metabolism， thereby improving psoriatic lesions.
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ObjectiveTo analyze the HIV infection characteristics and influencing factors among the spouses of HIV/AIDS patients in Shanghai. MethodsA cross-sectional survey was conducted to collect demographic， behavioral， epidemiological and spousal HIV detection information of newly reported and married patients with HIV/AIDS in Shanghai from January 2018 to July 2022 in the comprehensive HIV prevention and control information system of Chinese Center for Disease Prevention and Control. Descriptive analysis was used to analyze the HIV positivity rate of the spouses of HIV patients and the influencing factors were analyzed by univariate and multivariate logistic regression models. ResultsA total of 1 233 subjects were investigated， and the first HIV-testing positivity rate of the spouses of HIV/AIDS patients was 29.3% （361/1 233）. There were statistically significant differences in the HIV-testing positivity rate among spouses of HIV/AIDS patients by different age， gender， education level， occupation， transmission route， quantity of non-marital sexual activities， quantity of homo-sexual activities， and baseline CD4 cell count level （P<0.05）. Spouses of the HIV/AIDS patients aged ≥65 years old， female， heterosexual transmission， less non-marital sex， and no history of homosexual sex had relatively high HIV positive rate. The HIV-positive detection rate of spouses in the ≥65 age group was 1.81 times higher than that in the <45 age group. The HIV-positive detection rate of spouses in the female group was 3.66 times higher than that in the male group， and the HIV-positive detection rate of spouses in the homosexual transmission group was 0.25 times higher than that of the heterosexual transmission group. ConclusionRisk awareness of HIV infection among married people with spouses should be improved. The key populations with the characteristics such as females as the first HIV-positive reporter， and heterosexual transmission should be paid special attention.Their spouses should be mobilized to conduct HIV-testing as early as possible
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Objective:To investigate the relationship between serum microRNA-222(miR-222) and metabolism of glucose and lipid in women with polycystic ovary syndrome(PCOS).Methods:A total of 100 patients with PCOS (PCOS group) were enrolled from July 2018 to July 2020 in Puren Hospital Affiliated of Wuhan University of Science and Technology, and another 85 sterility patients were selected as control group. The levels of total cholesterol (TC), triglycerides (TG), high density lipoprotein cholesterol (HDL-C), low density lipoprotein (LDL-C), fasting insulin (FINS), fasting plasma glucose (FPG), insulin resistance index (HOMA-IR) as well as serum miR-222 were compared between two groups, and the correlation between serum miR-222 and metabolism of glucose and lipid was analyzed.Results:The levels of TC, TG, LDL-C, FPG, FINS, HOMA-IR, miR-222 in PCOS group were higher than those in the control group: (1.30 ± 0.25) mmol/L vs. (1.04 ± 0.21) mmol/L, (4.36 ± 0.91) mmol/L vs. (3.67 ± 0.61) mmol/L, (2.51 ± 0.89) mmol/L vs. (2.13 ± 0.42) mmol/L, (5.34 ± 1.56) mmol/L vs. (4.91 ± 0.84) mmol/L, (11.08 ± 3.83) mU/L vs. (7.49 ± 2.53) mU/L; the level of HDL-C in PCOS group was lower than that in the control group: (1.08 ± 0.47) mmol/L vs. (1.53 ± 0.50) mmol/L, and the differences were statistically significant ( P<0.05). The results from Logistic regression analysis showed that the levels of TC, TG, HDL-C, LDL-C, FINS, HOMA-IR, miR-222 were independent risk factors for abnormal of metabolism of glucose and lipid of polycystic ovary syndrome patients ( P<0.05). The correlation analysis showed that miR-222 expression had positive correlation with TC, TG, LDL-C, FINS, HOMA-IR ( r = 0.760, 0.737, 0.769, 0.749, 0.825, P<0.05), and had negative correlation with HDL-C( r = -0.743, P<0.05). Conclusions:The serum miR-222 shows abnormal increase in the PCOS patients, and is closely related to glucose and lipid metabolism. It can be used as a biomarker of abnormal glucose and lipid metabolism.
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Objective:To explore the effect of diversified nursing rehabilitation exercise in patients with SchatzkerⅡtibial plateau fracture.Methods:From January 2018 to December 2018, a total of 72 patients with Schatzker Ⅱtibial plateau fractures in Wuzhou traditional Chinese medicine hospital were selected by cluster sampling method for study. All patients were treated surgically. 36 of them were included in the control group (routine functional exercise after operation) by random number table method, and the remaining 36 patients were included in the observation group (on the basis of the control group, multiple functional rehabilitation exercise mode was given).The length of hospital stay, knee joint swelling degree and movement angle at different time after operation, preoperative and postoperative Harris Hip Score (HSS) and satisfaction were analyzed.Results:The time of fracture healing and hospitalization in the observation group were (92.58±20.65) d, (8.40±2.32) d, shorter than (108.56±25.38) d, (15.86±2.80) d in the control group ( tvalues were 2.930, 12.309, P<0.05). At 2 weeks and 6 weeks after operation, the swelling degree of knee joint in the observation group was (1.42±0.58) cm and (0.52±0.25) cm, which was less than (1.86±0.62) cm and (0.78±0.34) cm of the control group, respectively, the difference between the two groups was statistically significant ( tvalues were 3.110, 3.697, P<0.05); there was no significant difference in HSS between the two groups ( P>0.05); the score of observation group was (62.36±6.85), (70.32±5.69) points, which was significantly higher than (58.42±6.02), (63.45±6.28) points of control group ( tvalues were 2.592, 4.864, P<0.05); there was no significant difference in HSS score between the two groups 6 months after operation ( P>0.05). The satisfaction of the observation group was 97.22% (35/36), which was higher than 83.33% (30/36) of the control group, and the difference between the two groups was statistically significant ( χ 2value was 3.956, P<0.05). Conclusion:For Schatzker type Ⅱ tibial plateau fracture patients, taking diversified nursing rehabilitation exercise can quickly relieve their limb swelling, promote the recovery of joint function and fracture healing, make them recover as soon as possible, and improve their satisfaction.
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Objective@#To explore the correlation between serum visfatin level and cardiovascular disease risk factors in patient with polycystic ovary syndrome (PCOS).@*Methods@#A total of 100 PCOS patients (PCOS group) and 50 healthy women (control group) in Wuhan Puren Hospital from July 2016 to July 2018 were selected. In addition, according to the body mass index (BMI), the PCOS patients were divided into obese group (BMI ≥ 25 kg/m2, 57 cases) and non-obese group (BMI < 25 kg/m2, 43 cases). Serum level of visfatin was measured by enzyme-linked immunosorbent assay (ELISA) method, and the correlation between serum visfatin and follicle-stimulating hormone (FSH), luteinizing hormone (LH), testesterone (T), high sensitivity C-reactive protein (hs-CRP), blood lipids and blood glucose was analyzed.@*Results@#Compared with those in control group, the visfatin, BMI, diastolic blood pressure, waist and hip rate, hirsutism F-G score, FSH, LH, T, triglyeride (TG), fasting insulin (FINS), homeostasis model of assessment for insulin resistance index (HOMA-IR) in PCOS group were significantly increased: (103.20 ± 19.15) μg/L vs. (29.56 ± 4.14) μg/L, (25.81 ± 4.17) kg/m2 vs. (21.29 ± 3.74) kg/m2, (81.91 ± 10.32) mmHg (1 mmHg = 0.133 kPa) vs. (68.29 ± 7.57) mmHg, 0.91 ± 0.14 vs. 0.82 ± 0.13,(13.41 ± 2.91) scores vs. (5.98 ± 1.01) scores, (12.78 ± 1.98) U/L vs. (11.95 ± 1.51) U/L, (15.84 ± 3.16) U/L vs. (12.94 ± 1.53) U/L, (2.98 ± 0.32) nmol/L vs. (1.17 ± 0.20) nmol/L, (1.89 ± 0.43) mmol/L vs. (1.21 ± 0.11) mmol/L, (18.40 ± 2.98) mU/L vs. (11.23 ± 2.10) mU/L, 4.25 ± 0.86 vs. 2.51 ± 0.55; the high density lipoprotein-cholesterol (HDL-C) and ISI were significantly decreased: (1.46 ± 0.23) mmol/L vs. (1.55 ± 0.27) mmol/L and 0.011 ± 0.002 vs. 0.019 ± 0.005, and there were statistical differences (P<0.01 or <0.05). In addition, compared with those in non-obese group, the visfatin, BMI, diastolic blood pressure, hirsutism F-G score, FSH, LH, T, hs-CRP and TG in obese group were significantly higher, the HDL-C in obese group were significantly lower, and there were statistical differences (P<0.01 or <0.05). In PCOS group, serum visfatin level was positively correlated with BMI, diastolic blood pressure, hirsutism F-G score, FSH, LH, T, hs-CRP and TG, and serum visfatin level was negatively correlated with HDL-C (P<0.05). Stepwise multiple linear regression analysis showed that diastolic blood pressure and TG increased were the main predictors of increased visfatin level in patients with PCOS (P < 0.05).@*Conclusions@#Serum visfatin is increased in the patients with PCOS, and serum visfatin is positively related with the cardiovascular risk factors.
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Objective:To detect the levels of serum soluble semaphorin 4D in patients with type 2 diabetes, and to investigate the predictable value for diabetic microangiopathy (DMAP).Methods:A total of 210 type 2 diabetes patients were divided into non-DMAP group (96 cases), single-DMAP group (77 cases) and composite DMAP group (combination of two or three DMAP, 37 cases) according to the DMAP status. The general clinical data and laboratory indicators were collected, meanwhile, the levels of serum soluble semaphorin 4D were measured by enzyme-linked immunosorbent assay. The risk factors related to DMAP were analyzed by multiple ordered Logistic regression model, and the predictable value of serum soluble semaphorin 4D for DMAP was evaluated by receiver operating characteristic (ROC) curve.Results:The difference of course of diabetes [(5.02 ±1.00), (6.26 ± 2.13), (8.42 ± 2.82) years], systolic blood pressure (SBP)[(131.81 ± 18.06), (141.81 ± 22.51), (142.88 ± 17.06) mmHg, 1 mmHg=0.133 kPa], hypertension ratio [37.5%(36/96), 50.6%(39/77), 35.1%(22/37)], postprandial blood glucose (PPG)[(13.56 ± 2.02), (14.94 ± 2.50), (15.88 ± 2.45) mmol/L], hemoglobin (HbA 1c) [(8.17 ± 2.17)%, (9.14 ± 1.51)%, (9.69 ±1.58)%], soluble semaphorin 4D [(1.18 ± 0.27), (1.89 ± 0.53), (2.21 ± 0.79) μg/L] among non-DMAP group, single-DMAP group, composite DMAP group had statistical significance ( P<0.05). The levels of diabetes course, SBP, hypertension ratio, PPG, HbA 1c and serum soluble semaphorin 4D were significantly increased in the single-DMAP group and composite DMAP group compared to those in the non-DMAP group, the levels of diabetes course, PPG and serum soluble semaphorin 4D were significantly increased in the composite DMAP group compared to those in the non-DMAP group, and the differences were statistically significant ( P<0.05). Logistic regression analysis showed that serum soluble semaphorin 4D, diabetes course, hypertension ratio, PPG was independent risk factor for the DMAP ( P<0.05). ROC curve demonstrated that serum soluble semaphorin 4D>1.52 μg/L (AUC=0.873) may indicate the occurrence of single DMAP, whereas serum soluble semaphorin 4D>1.91μg/L (AUC=0.740) may predictethe occurrence of composite DMAP. Conclusions:Serum soluble semaphorin 4D is an independent risk factors for DMAP and may be considered as an potential serum maker to screen DMAP.
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Objective To explore the correlation between serum visfatin level and cardiovascular disease risk factors in patient with polycystic ovary syndrome (PCOS).Methods A total of 100 PCOS patients (PCOS group) and 50 healthy women (control group) in Wuhan Puren Hospital from July 2016 to July 2018 were selected.In addition,according to the body mass index (BMI),the PCOS patients were divided into obese group (BMI ≥ 25 kg/m2,57 cases) and non-obese group (BMI ＜ 25 kg/m2,43 cases).Serum level of visfatin was measured by enzyme-linked immunosorbent assay (ELISA) method,and the correlation between serum visfatin and follicle-stimulating hormone (FSH),luteinizing hormone (LH),testesterone (T),high sensitivity C-reactive protein (hs-CRP),blood lipids and blood glucose was analyzed.Results Compared with those in control group,the visfatin,BMI,diastolic blood pressure,waist and hip rate,hirsutism F-G score,FSH,LH,T,triglyeride (TG),fasting insulin (FINS),homeostasis model of assessment for insulin resistance index (HOMA-IR) in PCOS group were significantly increased:(103.20 ± 19.15) μg/L vs.(29.56 ± 4.14) μg/L,(25.81 ± 4.17) kg/m2 vs.(21.29 ± 3.74) kg/m2,(81.91 ± 10.32) mmHg (1 mmHg =0.133 kPa) vs.(68.29 ± 7.57) mmHg,0.91 ± 0.14 vs.0.82 ± 0.13,(13.41 ± 2.91) scores vs.(5.98 ± 1.01) scores,(12.78 ± 1.98) U/L vs.(11.95 ± 1.51) U/L,(15.84 ± 3.16) U/L vs.(12.94 ± 1.53) U/L,(2.98 ± 0.32) nmol/L vs.(1.17 ± 0.20) nmol/L,(1.89 ± 0.43) mmol/L vs.(1.21 ± 0.11) mmol/L,(18.40 ± 2.98) mU/L vs.(11.23 ± 2.10) mU/L,4.25 ± 0.86 vs.2.51 ± 0.55;the high density lipoprotein-cholesterol (HDL-C) and ISI were significantly decreased:(1.46 ± 0.23) mmol/L vs.(1.55 ± 0.27) mmol/L and 0.011±0.002 vs.0.019 ± 0.005,and there were statistical differences (P ＜ 0.01 or ＜ 0.05).In addition,compared with those in non-obese group,the visfatin,BMI,diastolic blood pressure,hirsutism F-G score,FSH,LH,T,hs-CRP and TG in obese group were significantly higher,the HDL-C in obese group were significantly lower,and there were statistical differences (P＜ 0.01 or ＜ 0.05).In PCOS group,serum visfatin level was positively correlated with BMI,diastolic blood pressure,hirsutism F-G score,FSH,LH,T,hs-CRP and TG,and serum visfatin level was negatively correlated with HDL-C (P ＜ 0.05).Stepwise multiple linear regression analysis showed that diastolic blood pressure and TG increased were the main predictors of increased visfatin level in patients with PCOS (P ＜ 0.05).Conclusions Serum visfatin is increased in the patients with PCOS,and serum visfatin is positively related with the cardiovascular risk factors.
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Objective@#To investigate the association between maternal nocturnal sleep during pregnancy and their infants′ nocturnal sleep patterns in early infancy by establishing a birth cohort.@*Methods@#In this prospective study,healthy pregnant women and their infants were recruited in Chongqing Health Center for Women and Children from September 2013 to January 2014. A total of 40 healthy pregnant women and their infants were enrolled, and 34 pairs (85%) completed the whole survey. Actiwatch, wearing on the mother′s wrist or infant′s ankle,was used to monitor nighttime sleep at home for 7 times in total,with 7 consecutive days for each monitoring course. Pregnant women were monitored at 16, 24, 32 weeks gestational age, and were required to finish Pittsburgh Sleep Quality Index (PSQI) scale at the end of each monitoring. Their infants were initially monitored at 14 days after birth, followed by 1st, 2nd, and 3rd month after birth,with sleep diary recorded by parents. Pearson correlation analysis and multivariate linear regression were used to investigate the potential correlation between these two sleep patterns.@*Results@#The average nocturnal sleep time was (465±53) min throughout pregnancy, with increased trend in the latency of maternal nighttime sleep onset ((30±15), (34±29), (38±30) min) while decreasing of average sleep efficiency ((85±5)%, (84±8)%, (83±8)%). The longest night sleep duration decreased from (382±74) min in the first trimester to (330±83) min in the third trimester (F=4.932, P<0.05), while awakening time after sleep onset (WASO) correspondingly increased from (27±22) min to (53±25) min (F=12.605, P<0.05). In terms of infants, the latency of nighttime sleep onset decreased from (43±33) min on the 14th day to (20±29) min at 3-month of age (t=3.281, P=0.002), while the longest nighttime sleep duration increased from (20±62) min to (265±140) min (t=3.867, P<0.01); meanwhile, the total nocturnal sleep time and the sleep efficiency increased from (373±126) min and (63±28)% to (431±103) min and (75±16)%, respectively (t=2.362, P=0.024; t=2.418, P=0.039, respectively). After adjusting for maternal age and body mass index (BMI), parental educational level and family income, maternal mood, exposure to tobacco and alcohol during pregnancy, method of delivery, as well as infants′ gender, feeding patterns and gestational weeks, the regression analysis showed that the total nocturnal sleep time and the longest nighttime sleep duration during pregnancy were positively correlated with the corresponding parameters of their infants at the age of 3 months (B=0.541, 95%CI 0.168-0.914, t=2.882, P=0.005; B=0.310, 95%CI 0.035-0.586, t=2.240, P=0.027, respectively).@*Conclusions@#The total nocturnal sleep time and the longest nighttime sleep duration during pregnancy are positively correlated with the corresponding parameters of their infants in early infancy. Paying more attention to improve maternal sleep quality may facilitate their infants to establish sleep-wake patterns early.
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Objective To evaluate the application value of quantitative immune fecal occult blood test (FOBT) in colonoscopy for the screening of colorectal cancer in health check-up participants. Methods The subjects were selected from July 2017 to June 2018 in the Health Management Center of the Second Affiliated Hospital of Suzhou University. The subjects were the healthy individuals who chose quantitative immune FOBT or chemical method plus immunogold double-method FOBT (referred to as"double-method FOBT"), excluding those who had interfering factors. Individuals with a positive result in primary screening were selected and conducted with colorectal cancer by colonoscopy. If the polyploidy lesions were observed during colonoscopy, the biopsy or excision was performed, and the pathological diagnosis was performed. The positive rate of primary screening, compliance rate of colonoscopy and pathological results of colonoscopy were compared between the two methods. Quantitative immunoassay FOBT was analyzed in different gender, age group, physical examination nature, positive rate of primary screening, compliance rate of colonoscopy and pathological results of colonoscopy. Results 18 728 people chose quantitative immunoassay FOBT and 6 212 people chose double-method FOBT at the same time. There was no significant difference in gender and age between the two groups (all P>0.05), which was comparable. The detection rate of quantitative immune FOBT was higher than double-method FOBT (74.62% vs 32.23%, P<0.001). The positive rate of quantitative immune FOBT in primary screening was lower than double-method FOBT (4.11% vs 5.34%, P=0.003). The colonoscopy screening rate in positive population by quantitative immune FOBT was higher than double-method FOBT (27.83% vs 13.08%, P=0.001). These differences were statistically significant. The detection rate of total lesions by colonoscopy was 71.88% in positive population by quantitative immune FOBT. It was 42.86% in double-method FOBT. There was no statistical difference between the two methods (P=0.05). The detection rates of quantitative immune FOBT were significantly different among different genders, ages and physical properties (all P<0.001). The detection rate was higher in males than in females (79.14% vs 68.75%). The detection rate was highest in the group between 40 and 59 years old (79.96%). The individual detection rate was higher than the group (90.08% vs 66.07%). The positive rates in primary screening were significantly different among different ages (P=0.001).It was highest in the group aged 60 or above (5.59%). The colonoscopy screening rate in positive population by quantitative immune FOBT was highest in the group aged 50 or above (36.96%). The detection rate of inflammatory lesions were significantly different among different ages (P<0.001). The detection rate of colorectal cancer in males was higher than in females (11.11% vs 0.00%, P=0.009). In addition, with the increasing of fecal occult blood value, the detection rate of cancer was increased (P=0.041). Conclusion The quantitative immune FOBT is an ideal non-invasive examination for early screening of colorectal cancer. It has important application values.
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Objective To investigate the influence of intervention method on infantile physical growth and sleep awakening mode and its effect on infantile sleep problem.Methods Six hundreds healthy infants aged 1 month old voluntarily receiving health mangement in this hospital fnom May to August 2014 were selected and divided into the intervention group and control group.The sleep questionnaire was filled.All infants monthly received the common child health care conventional guidance follow up until 6 months old.The intervention group conducted the sleep cognition education and sleep problem consultation by the sleep specialist physicians issuing the infantile sleep health care education propaganda leaflets,then the the infants with sleep problems conducted the comprehensive intervention according to the sleep hel ath prescription.Results In the comparison of the infantile physical growth indicators,sleep awakening mode indicators and sleep problem occurrence rate between the two groups:(1) the infantile body height in the intervention group had obvious difference compared with the control group(P＜0.05).(2)The night waking frequency,night waking time,daytime sleep frequency and daytime sleep total time in the intervention group were significantly lower than those in the control group(P＜0.05),the night total sleep time in the intervention group was increased compared with the control group(P＜0.05),and the sleep latency,time to falling asleep and daytime naps time had no statistical difference between the intervention group and control group(P＞0.05).(3) In the sleep problems at 6 months old,the intervention group was significantly lower than the control group in the aspectof excessive night waking(P＜0.05),but the aspects of falling asleep overtime,awakening/going to bed rhythm disorder had no obvious difference between the two groups.Conclusion The early intervention strategy of cognitive behavioural intervention and health education can generate the favorable effect on the physical growth and sleep awakening mode in 0-6 months old infants and can reduce the occurrence of infantile sleep problems.
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<p><b>OBJECTIVE</b>To study the clinicopathologic characteristics of IgG4-related sialodacryoadenitis and chronic rhinosinusitis (CRS).</p><p><b>METHODS</b>A total of 13 patients (patient group) were evaluated clinically and biopsy specimens from the lacrimal/salivary glands (n=12) and nasal mucosa (n=8) were reviewed and immunohistochemistry was performed to assess IgG-and IgG4-positive cells. Similarly, nine patients with IgG4-related sialodacryoadenitis without CRS and 10 patients with common CRS were included as controls.</p><p><b>RESULTS</b>There were 8 male patients and 5 female patients. The age of patients ranged from 32 to 71 years (mean 50.2 years). The patient group had higher serum IgG4 concentration than that of the control group (P<0.05). Lymphoplasmacytic infiltration, lymphoid follicle formation and sclerosis were prominent in lacrimal/salivary glands in both groups; however the magnitude of IgG4-positive plasmacytic infiltration in the patient group was significantly higher than that of the control group (P<0.05). Similarly, evaluation of nasal mucosa revealed greater lymphocytic and plasmacytic infiltration, and lymphoid follicle formation, together with significantly higher amount of IgG4-positive plasma cell infiltration in the patient group compared to the common CRS group (P<0.05).</p><p><b>CONCLUSIONS</b>IgG4-related disease (IgG4-RD) simultaneously involving lacrimal/salivary glands and nasal cavity/paranasal sinuses is rare and characterized by a combination of IgG4-positive plasma cell infiltration involving lacrimal/salivary glands and nasal mucosa along with an increased serum level of IgG4. As a systemic disease, early and accurate diagnosis is therefore of great importance, and unnecessary surgery should be avoided.</p>
Subject(s)
Adult , Aged , Female , Humans , Male , Middle Aged , Chronic Disease , Immunoglobulin G , Blood , Immunohistochemistry , Lacrimal Apparatus , Pathology , Nasal Mucosa , Pathology , Paranasal Sinuses , Pathology , Rhinitis , Diagnosis , Allergy and Immunology , Salivary Glands , Pathology , Sialadenitis , Diagnosis , Allergy and Immunology , Sinusitis , Diagnosis , Allergy and ImmunologyABSTRACT
Objective To investigate and analyze the situation of urban neglected children aged 3 to 6 in 9 districts of Chongqing .Methods Totally 1 316 children were randomly sampled under stratification from 18 streets of 9 district of Chongqing . Based on the Child Neglect Norms used by China ,prevalence of child neglect was identified and SPSS 13 .0 was employed for statis‐tical analysis .Scores ,frequency/degrees ,age ,sex and 5 types (physical ,emotional ,educational ,safety and medical) of child neglect on every group of the regions were calculated .Results The average prevalence of child neglect for the 3 to 6 year‐olds was 22 .95% ,and the total degree was 39 .56 ± 7 .19 .No significant differences were found in the prevalence and degree of child neglect between males and females and among age groups (P<0 .05) .The frequencies of child neglect for the five types were 5 .09% to 10 .64% ,with the higher frequencies of safety neglect (10 .64% ) and physical neglect (9 .50% ) .The degrees of child neglect for the five types were 36 .94 to 41 .24 ,with the higher degrees of educational neglect and physical neglect (41 .24 ± 10 .43 ,39 .81 ± 9 .32 respectively ) .No significant differences were found in the frequency of the types between males and females and among age groups . No significant differences were found in the degree of the types (with an exception on emotional neglect among age groups) between males and females and among age groups .The children aged 3 to 6 were mainly involved in single item of negligence ,with incidence rates as 13 .68% and proportions as 59 .60% .Conclusion The frequency and degree of child neglect among children aged 3 to 6 in the urban areas of 9 district of Chongqing were medium ,and similar between males and females and among age groups ,except the degree of emotional neglect .The children aged 3 to 6 had the higher frequencies of safety and physical neglect ,and the higher de‐grees of educational and physical neglect .The children aged 3 to 6 were mainly involved in single item of negligence .
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With the continuous development of the reform of the state marketing economic system and the health care service system, the medical scientific research institutions have paid much more attention to the innovation of operating managerial patterns in practice. As an important and advanced managerial patter in thought and theoretical aspects, the construction of enterprise culture has played a major role in many institutions. In this paper, the general background, culture deposits and innovation in institutions were discussed;the ideas and actions in practice of culture innovation in new situation were proposed via practical activities taken place at the Institute.
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Objective: To investigate the effects of Liangxue Tongyu Formula (LXTYF), a compound traditional Chinese herbal medicine, on brain edema in rats with intracerebral hemorrhage and to explore the mechanism. Methods: Intracerebral hemorrhage was induced by using the intrastriatal autologous blood injection. Rats were randomized into sham-operated (SO) group, intracerebral hemorrhage (ICH) group and LXTYF group. Rats in the LXTYF group were intragastrically administered with LXTYF every day while the other two groups were given normal saline. Brain water content was determined at 24, 48, 72, and 120 h after intracerebral hemorrhage. Matrix metalloproteinase-9 (MMP-9) level, and MMP-9 and tissue inhibitor of metalloproteinase-1 (TIMP-1) mRNA expressions in perihematoma area were detected by gelatin zymography and fluorescence quantitative real-time polymerase chain reaction at the four time points, respectively. Results: Water content in the ICH group was highly elevated after intracerebral hemorrhage, and reached to the peak at 72 h. Compared with the ICH group, the LXTYF group had lower water contents at 48, 72 and 120 h after intracerebral hemorrhage (P<0.01). The difference in water content between the LXTYF and SO groups was significant only at 72 h (P<0.01). Although the pro-MMP-9 level and MMP-9 activity in the LXTYF and ICH groups were enhanced, they were still lower in the LXTYF group than in the ICH group (P<0.01 for 24, 48, 72 and 120 h, respectively). And there was no significant difference in them between the LXTYF group and the SO group at 120 h. Meanwhile, MMP-9 mRNA expressions were increased in the ICH and LXTYF groups, but the levels in the LXTYF group were significantly lower (P<0.01 for 48, 72 and 120 h, respectively) than those in the ICH group. Also, TIMP-1 mRNA expressions at 24, 48, 72 and 120 h after intracerebral hemorrhage were up-regulated in the LXTYF group, and there were significant differences in TIMP-1 expressions between the LXTYF group and ICH group after intracerebral hemorrhage (P<0.01). Conclusion: Liangxue Tongyu Formula ameliorates brain edema in rats after intracerebral hemorrhage by inhibiting MMP-9 expression and activity and up-regulating TIMP-1.
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OBJECTIVE: To observe the therapeutic effects of Yiqi Sanju Formula (YQSJF), a compound Chinese herbal medicine, on central obese men at high risk of metabolic syndrome (MS). METHODS: Compared with 30 healthy male volunteers, 45 central obese men were separated randomly into two groups and received the interventions with YQSJF and placebo respectively for 10 weeks. Baseline characteristics, insulin resistance, inflammation cytokines and plasminogen activator inhibitor-1 (PAI-1) and tissue plasminogen activator (t-PA) were evaluated before and after treatment. RESULTS: The score of homeostatic model assessment for insulin resistance (HOMA-IR) and the levels of C reactive protein (CRP), free fatty acid (FFA) and PAI-1 in obese men were higher than those in the control group, while t-PA was lower. After treatment, compared with placebo group, body mass index, waist, and waist-to-hip ratio were decreased significantly in subjects who received YQSJF (P<0.01). The score of HOMA-IR and the levels of CRP, FFA and PAI-1 were decreased significantly, and the level of t-PA was increased significantly (P<0.01). CONCLUSION: YQSJF can reduce obesity and insulin resistance in central obese men at high risk of MS and improve inflammation and fibrinolysis, which indicates that it can reduce the risk of atherosclerosis.
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Objective To compare the hemodynamic responses to orotracheal intubation during emergency operation with the proseal laryngeal mask airway and endotracheal Intubation.Methods Fifty six patients who were ASA GRADE Ⅰ-Ⅱ before onset undergoing orotracheal intubation were randomly dirideed into either without laryngoscope direct proseal laryngeal mask intubation(PLMA) group or direct laryngoscope endotracheal intubation(TT) group.SBP、DBP、HR and SpO2 were recorded before,durring and 5 minutes after intubation.Intubation time and first attempt insertion success rate were recordeddso.All patients were followed up postoperatively for adverse effects like sore throat or hoarseness.Results In intubation time、first attempt insertion success rate and adverse effects like sore throat or hoarseness,PLMA group was 96.43%,(19.7?5.7)s and 7.1%,TT group was 100%,(27.9?11.3)s and 39.29%.Intubation time was significantly shorter and adverse effects like sore throat or hoarseness were lower in the PLMA group than TT group(P
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OBJECTIVE To investigate the relationship of obstructive sleep apnea hypopnea syndrome (OSAHS) and hyperglycemia by analyzing the level of blood-fasting sugar (BFS) in patients with OSAHS. METHODS The data of BFS and AHI in patients with OSAHS (n=300) and the data of BFS of controls (n=225) were collected. The difference of high blood glucose in the OSAHS and the controls.and the difference of AHI between OSAHS patients with high blood glucose and OSAHS patients with normal blood glucose was analyzed. RESULTS There were 97 patients (32.3 %) with high BFS in 300 OSAHS patients, but only 14 cases (6.2 %) with high BFS in 225 controls. There was a significant difference between two groups. The average of AHI in OSAHS patients with high BFS was 67.8/h, and average of AHI in OSAHS patients with normal BFS was 38.4/h. There was a significant difference between the two groups. CONCLUSION There is a close correlation of OSAHS and hyperglycemia.
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OBJECTIVE: To observe the effects of Pollen Typhae total flavone (PTF) on glucose and lipid metabolism in 3T3-L1 adipocytes. METHODS: The content of glucose which disappeared from the culture medium after incubation with drugs for 24 hours was determined as glucose consumption of the cells. The activity of cells was detected by XTT method. The transport of glucose was observed by (3)H-glucose uptake method. The efflux of free fatty acid (FFA) from adipocytes was observed by the concentration of FFA in the culture medium. RESULTS: The glucose concentration in culture medium was significantly decreased with a concentration-dependent effect, when PTF concentrations were from 0.025 g/L to 0.4 g/L. The toxic effect on cells appeared while PTF concentration was 0.4 g/L, and the MTT value decreased. PTF also significantly increased glucose transportation in the 3T3-L1 adipocytes as rosiglitazone (ROS) did. At the same time, FFA concentration in culture medium was significantly decreased as compared to the normal control group, while ROS-treated group did not show any difference. CONCLUSION: PTF can increase insulin sensitivity by increasing glucose transportation and consumption in the 3T3-L1 adipocytes as well as decreasing the FFA efflux from the cells.
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BACKGROUND: Acute coronary syndrome(ACS) are a result of the rupture of unstable atherosclerotic plaques and thrombosis. Matrix metalloprotei nases (MMPs) are believed to play an important role in vascular remodeling and plaque instability by degrading the extracellular matrix.OBJECTIVE: To investigate the possible impact of polymorphisms in matrix metalloproteinase-3 and -12 genes on the risk of ACS in Chinese Han population.DESIGN: A case-control study based on ACS patients as subjects and healthy population as the control group.SETTING: Department of clinical tests of a university hospital and the department of biochemistry and molecular biology of a medical college PARTICIPANTS: A total of 103 patients with ACS(outpatients and inpatients received and hospitalized from March 2002 to December 2003 at the People' s Armed Police Hubei General Hospital,Zhongnan Hospital of Wuhan University and Liyuan Hospital of Huazhong University of Science and Technology) were involved and were divided into ACS group and the normal control group. For the ACS group,there were 73 males and 30 females with an average age of(53±10) years,including 47 cases of acute cardiac infarction and 56 cases of unstable cardiac angina. For the normal control group,there were 100 healthy subjects as controls,including 67 males and 33 females with an average age of(52±12) years. All the subjects were Chinese Han Population in Hubei Province,and they were voluntary to participate in the study.METHODS: Polymerase chain reaction(PCR) and restriction fragment length polymorphism(RFLP) techniques were used to examine the MMP-3-1612 5A/6A and MMP-12 -82 A/G gene polymorphisms respectively.MAIN OUTCOME MEASURES: The genotypes of and the allele frequencies in the patients and the individuals in the control group.RESULTS: The prevalence of 5A/6A +5A/5A genotype(41.7% vs 24%,x2 =6. 447,P<0.05) and5A allele (21.8% vs 13%,χ2 =4. 906,P<0.05) of MMP-3 gene were significantly more frequent in the ACS group than in the control subjects. The odds ratio of the 5A/6A +5A/5A was 2. 269(95% CI,1.241 to 4. 149,P<0.01). No significant difference was detected between the patients and the control group in genotype and allele distribution of the MMP-12 -82 A/G gene polymorphism(P>0.05).CONCLUSION: These findings indicate that the 5A/6A polymorphism of MMP-3 gene is associated with the risk of ACS,and might play an important role in the development of coronary atherosclerosis..This conclusion provides experimental data for gene study of the coronary disease and primary intervention for rehabilitation.
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<p><b>OBJECTIVE</b>To assess the atherogenicity of lipoprotein(a), the effect of the heterogeneity of lysine binding of apolipoprotein(a) [apo(a)], a plasminogen-like glycoprotein component on the proliferation of human arterial smooth muscle cells (SMCs).</p><p><b>METHODS</b>Both wild type (wt) Trp72 and mutant (mut) Trp72-->Arg forms of apo(a) kringle IV-10 were expressed by employing a GST-gene fusion system into E. coli. The proliferation of SMCs was determined by flow cytometry and MTT colorimetry. Enzyme-linked immunosorbent assay (ELISA) assay was used to detect the active form of transforming growth factor beta(1) (TGF-beta(1)).</p><p><b>RESULTS</b>Apo(a) wt-kringle IV-10 that has lysine binding properties possessed a growth-stimulating activity to SMCs on a dose-dependence manner by stimulating cells in the G(1)/G(0) phase of cell cycle to S and G(2)/M phase, and reduced significantly the amounts of endogenous active TGF-beta(1) in culture when compared with the control medium and the GST group (2.4 +/- 0.5 vs 8.6 +/- 1.6 and 9.1 +/- 1.7 ng/ml, P < 0.01). The growth-stimulating effect of apo(a) mut-kringle IV-10 deficient in lysine binding was negligible.</p><p><b>CONCLUSIONS</b>Apo(a) induces SMCs growth by inhibiting the activation of latent TGF-beta(1), an activity that may involve the ability of apo(a) kringle IV-10 to bind lysine. The mitogenic effect of apo(a) wt-kringle IV-10 on SMCs might play an active role in the atherogenic function of lipoprotein(a).</p>